Function reference • topcorneR

Plate functions

Functions working on Plate objects

head(<Plate>) as.character(<Plate>) show(<Plate>): A multiwell plate

PlateTypeToWellNames(): Well names of plate type

as.character(<Well>) show(<Well>) Row() Column(): The row of a well

nextWell(): Move to the next well

plateMap() plateMap_all(): Plot the contents of a plate

plateWellVolume(): Get reagent volume

randomise(): Randomise reaction coordinates

seekReagent(): Find a well that can provide enough reagent

setWell(): Set the contents of a well

set_block(): Set values in rectangular areas of a plate

sourceReagent(): Get reagent name

Well functions

Functions working on Well objects

PlateTypeToWellNames(): Well names of plate type

as.character(<Well>) show(<Well>) Row() Column(): The row of a well

Well-class Well: Well coordinates

nextWell(): Move to the next well

validRow() validColumn(): Tests the well's row name is valid

qPCR functions

Functions to analyse qPCR results, including convenience wrappers to the qpcR package.

calc_qPCR_TM(): Compute melting temperature from raw data

calc_qPCR_mod(): Compute CT and amplification efficiency from raw data

load_qPCR_results() load_qPCR_meltcurve() load_qPCR_raw(): Load CT values from qPCR results file

Example data

exampleEcho: Example Echo object

examplePlate: Example Plate object

exampleTransducer: Example Transducer object

Dispenser functions

Dispenser functions

as.character(<Echo>) show(<Echo>): Echo state

as.character(<Transducer>) show(<Transducer>): Transducer state

changeWell(): Change a well position

moveTransducer(): Change the state of the transducer

planTransfers(): Plan Echo transfers

showLogs() exportLogs(): Show or export transfer logs

transfer(): Transfer volume from a source to a destination plate

Accessory functions

Accessory functions

N: Avogadro's constant

titration_curve(): Titration curve

Dispensing functions

dispense_pairs(): Dispense pair combinations

set_block(): Set values in rectangular areas of a plate

Private functions

bisectError(): Private function to search for defective wells