Loads CT values from the results file produced by a real-time quantitative PCR (RT-qPCR) machine.
Usage
load_qPCR_results(
file,
expName = NA,
machine = c(NA, "VIA", "QS5"),
format = "Excel",
skip = 43,
n_max = 384
)
load_qPCR_meltcurve(
file,
expName = NA,
machine = c(NA, "VIA", "QS5"),
format = "Excel",
skip = 42
)
load_qPCR_raw(
file,
expName = NA,
machine = c(NA, "VIA", "QS5"),
format = "Excel",
skip = 42,
cycles = 40
)Arguments
- file
Path to the file to open.
- expName
The name of the experiment.
- machine
The type of the machine that produced the results file.
- format
The format of the file. At the moment only Excel is supported.
- skip
The number of lines to skip before reading the data.
- n_max
The number of lines to read.
- cycles
The number of PCR cycles.
Value
Returns a tibble::tibble() object reporting well coordinates, CT
value and melting temperature as computed by the machine, and optionally
the name of the experiment.
See also
Other qPCR functions:
calc_qPCR_TM(),
calc_qPCR_mod()
Other qPCR functions:
calc_qPCR_TM(),
calc_qPCR_mod()
Other qPCR functions:
calc_qPCR_TM(),
calc_qPCR_mod()