Given a number of steps and a target maximal volume, this function will return an increasing serie of volumes that cover the range in an approximately logarithmic progression. Each volume can be dispensed, as they are guaranteed to be multiple of the droplet's volume. The range can be forced to start at zero if a negative control is needed.
Arguments
- steps
The number of points in the titration curve.
- maxvol
The maximum volume to reach.
- dropvolume
The volume of a droplet, in nanoliters.
- zero
Start with a negative control with a volume of zero.
Value
A numeric vector of length steps indicating the volumes to transfer
in order to cover a volume range in a logarithmic manner.
See also
Other Accessory functions:
N
Examples
titration_curve(12)
#> [1] 0.0 2.5 5.0 10.0 17.5 32.5 55.0 100.0 177.5 315.0
#> [11] 562.5 1000.0
titration_curve(12, zero = FALSE)
#> [1] 2.5 5.0 7.5 15.0 25.0 40.0 70.0 120.0 202.5 345.0
#> [11] 587.5 1000.0
titration_curve(12, dropvolume = 25)
#> [1] 0 25 50 75 100 125 175 250 350 500 700 1000
titration_curve(12, dropvolume = 25, maxvol = 300)
#> [1] 0 25 50 75 100 125 150 175 200 225 250 300