Given a source plate, a target plate and the representation of a filled target plate, calculate the liquid transfers necessary perform the filling.
Arguments
- source
A source
Plate.- destination
A destination
Plate.- plan
A
Platerepresenting the additions to the destination plate.- model
Which Echo model to use.
Details
Each reagent of the plan plate is transferred one after the other. Once a reagent is selected, a source well is sought for the transfer and the transducer is moved to it.
See also
Other Dispenser functions:
Echo-class,
Transducer-class,
changeWell(),
moveTransducer(),
showLogs(),
transfer()
Examples
sourcePlate <- examplePlate
head(sourcePlate)
#> DataFrame with 6 rows and 2 columns
#> dNTP buffer
#> <numeric> <numeric>
#> A01 1e+05 NA
#> A02 1e+05 NA
#> A03 NA 1e+05
#> A04 NA NA
#> A05 NA NA
#> A06 NA NA
planPlate <- Plate("384") |>
setWell(Well("A01"), "dNTP", 50) |>
setWell(Well("A02"), "dNTP", 100) |>
setWell(Well("A01"), "buffer", 50)
head(planPlate)
#> DataFrame with 6 rows and 2 columns
#> dNTP buffer
#> <numeric> <numeric>
#> A01 50 50
#> A02 100 NA
#> A03 NA NA
#> A04 NA NA
#> A05 NA NA
#> A06 NA NA
destinationPlate <- Plate("384", deadVolume = 1e4, maxVolume = 1e5)
echo <- planTransfers(sourcePlate, destinationPlate, planPlate)
echo
#> A 525 Echo machine:
#> Source plate: A Plate with data about 384 wells (dead volume: 10000; max volume: 1e+05).
#> Destination plate: A Plate with data about 384 wells (dead volume: 10000; max volume: 1e+05).
#> Transducer at the following coordinates:
#> Source: A03
#> Destination: A01
#> 3 elements in the log.
echo |> showLogs()
#> from to vol what
#> 1 A01 A01 50 dNTP
#> 2 A01 A02 100 dNTP
#> 3 A03 A01 50 buffer